• Українська
  • English
  • Русский
ISSN 2415-3400 (Online)
ISSN 1028-821X (Print)

Electromagnetic and acoustic technologies in antibacterial preparation development

Рубрика: 
APPLIED RADIOPHYSICS
Kovalenko, OI, Kalinichenko, SV, Babich, EM, Kivva, FV, Roenko, OM, Antusheva, TI
Organization: 

1 O.Ya. Usikov Institute for Radiophysics and Electronics NASU
12, Acad. Proskury St., Kharkiv, 61085, Ukraine

2 I. Mechnikov Institute of Microbiology and Immunology National Academy of Medical Sciences of Ukraine
14/16, Pushkins’ka St., Kharkov, 61057, Ukraine

E-mail: Kovalenko-ire@ukr.net
https://doi.org/10.15407/rej2021.04.040
Язык: english
Аннотация: 

Subject and Purpose. The present paper is concerned with the use of wave technologies in the development of antibiotics-alternative approaches for pathogenic microflora suppression. Lactobacilli strains picked in different ecological niches and their activity against pathogenic strains are studied with a focus on a targeted modification of adhesive and antagonistic properties of lactobacilli by exposing them to low-intensity electromagnetic (EM) fields and the ultrasound.

Methods and Methodology. Lactobacilli picked in different ecological niches are experimentally studied, including (1) standard strains from probiotic preparations and (2) circulating strains picked in humans and bees. For the ultrasonic and electromagnetic radiation sources, G3-109 and G3-F and G4-141 and G4-142 generators are taken, respectively. The adhesive properties of Lactobacillus spp. strains and their antagonistic activity are estimated against C. diphtheriae, S. aureus and yeast-like fungi of Candida genus in aerobic and microaerophilic culture conditions. Statistical technology is employed in the data processing and analysis.

Results. It has been established that L. plantarum strains picked in the gut of healthy bees are most antagonistic towards pathogens. It has been demonstrated that the priority culture conditions for lactobacilli are microaerophilic conditions simulating their stay in vivo. It has been shown that it is possible to modify properties of microorganisms by their exposure to ultrasound and low-intensity electromagnetic fields in narrow bands of the EHF range. The effect efficiency versus frequency has a dispersion character. Individual features of various pathogenic strains have been recognized.

Conclusion. The obtained results open up prospects for electromagnetic and acoustic technologies in the development of safe alternative means to antagonize persisting pathogens and increase human body resilience.
Ключевые слова: adhesive and antagonistic activity, diphtheria, electromagnetic field, golden staphylococcus, lactobacilli, modification, ultrasound

Manuscript submitted 09.09.2021
Radiofiz. elektron. 2021, 26(4): 40-50
Full text (PDF)
 

 

References: 

 

1. Electromagnetic fields and human health. In: Proc. 2nd Int. Conf. Problems of human electromagnetic safety. Fundamental and applied research. EM field normalization: philosophy, criteria and harmonization. Moscow, Russian Federation, 20-24 Sept. 1999 (in Russian). Moscow, 1999.
 
2. Kolbun, N.D., Bessonov, A.E., and Volyanyuk, R.E., 1993. Information and wave therapy: scientific-practical guide. Kiev: Ukr. Encycl. Publ. (in Russian).
 
3. Kravkov, G.A., 2006. Effect of non-thermal (informational) effects of electromagnetic radiation of extremely high frequency on biological objects and humans. Part 1. Kiev. 123 p. (in Russian). Available from: https://ru.calameo.com/books/001038164cea38791e8b1
 
4. Zelentsov, V.I., Perel'muter, Ya.M., Cha, V.A., Fal'kovich, V.M., Votoropin, S.D., 2007. Biological effects of low intensity millimeter electromagnetic radiation. Millimeter Waves in Biology and Medicine, 2(46), pp. 42-49 (in Russian).
 
5. Perlmutter, D., Loberg, K., 2015. Brain Maker: The Power of Gut Microbes to Heal and Protect Your Brain - for Life. Publisher: Little, Brown Spark.
 
6. Valyshev, A.V., Gilmutdinova, F.G., 2006. Microbial Ecology of the Human Digestive Tract. In: O.V. Bukharin, ed. 2006. Ecology of Human Microorganisms. Yekaterinburg: Institute of Cellular and Intracellular Symbiosis Publ., pp. 169-290 (in Russian).
 
7. Volodin, N.N., Degtyareva, M.V., 2000. Role of pro- and anti-inflammatory cytokines in immune adaptation of newborn babies. Int. J. Immunorehabilit., 2, pp. 175-184 (in Russian).
 
8. Kartashova, O.L., Usvyatsov, B.J., 2006. Human skin microflora. In: O.V. Bukharin, ed. 2006. Ecology of Human microorganisms. Yekaterinburg: Institute of Cellular and Intracellular Symbiosis Publ., pp. 61-102 (in Russian).
 
9. Wenner, M., 2008. Going with his gut bacteria. Sci. Amer., 299(1), pp. 90-92. DOI: https://doi.org/10.1038/scientificamerican0708-90
 
10. Yankovsky, D.S., Dyment, G.S., 2008. Microflora and Human Health. Kiev: Chervona Ruta-Tours Publ. (in Ukrainian).
 
11. Kalinichenko, S.V., Korotkykh, O.O., Tishchenko, I.Yu., 2013. Current state of development and use of probiotic, prebiotic and sinbiotichny medicines (review). Annals of Mechnikov Institute, 3, pp. 5-12 (in Ukrainian). Available from: http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?Z21ID=&I...
 
12. Soloveva, I.V., Tochilina, A.G., Novikova, N.A., Belova, I.V., Ivanova, T.P., Sokolova, K.Ya., 2010. Study of biological properties of new lactobacillus strains. Vestnik of Lobachevsky State University of Nizhni Novgorod, 2(2), pp. 462-468 (in Russian).
 
13. Bengmark, S., 2003. Synbiotic treatment in Clinical Praxis. In: Host Microflora Crosstalk. Old Herborn University Seminar, 16, pp. 69-82.
 
14. Shenderov, B.A., 2005. Probiotics, prebiotics and synbiotics. General and selected sections of the problem. Food ingredients. Raw materials and additives, 2, pp. 23-26.
 
15. Maslyanko, R.P., 1999. Principles of Immunobiology. Lviv: Vertikal' Publ. (in Ukrainian).
 
16. Standardization of preparation of microbic suspensions: Information letter on innovations in health care system. 163. Kyiv: Ukrmedpatentinform, 2006. 10 p. (The normative document. Ministry of Health of Ukraine; Ukrainian center of scientific medical information and patent and license work. Information letter) (in Ukrainian).
 
17. Brilis, V.I., Brilene, T.A., Lentsner, H.P., Lentsner, A.A., 1986. Technique of studying of adhesive process of microorganisms. Laboratory business, 4, pp. 210-212.
 
18. Yegorov, N.S., 1965. Microbes-antagonists and biological methods of determining antibiotic activity. Moscow: Higher School Publ. (in Russian).
 
19. Borshch, S.K., Seredyuk, N.M., Kutsik, G.V., 2004. Studying of antagonistic activity of the lactobacilli entered into probiotichny medicine laktobakterin, etiologichesky factors of an intestinal dysbiosis, pyoinflammatory processes and probiotic strains of microorganisms. Galician Med. J., 3, pp. 16-19 (in Ukrainian).
 
20. Bendat, J., Pirsol, A., 1989. Applied random data analysis. Translated from English by V.E. Privalsky, A.I. Kogubinsky. Moscow: Mir Publ. (in Russian).
 
21. Popov, M.M., Kalinichenko, S.V., Chausovska, T.A., Babich, E.M., Korotky, A.A., Kivva, F.V., Kovalenko, O.I., Balak, A.K., 2016. Influence of electromagnetic radiation and gas composition of the atmosphere cultivation on ability of stafilokokk and korinebakteriya to bioplivkoutvorennya. Biomedical and biosocial anthropology, 26, pp. 45-49 (in Ukrainian).
 
22. Kalinichenko, S.V., 2005. Kalinichenko, S.V., 2005. Influence of millimeter waves on an allosteric regulation of enzymes of the corynebacteria associated with a cycle of trikarbonovy acids. Problems of uninterrupted medical training and science, 2, pp. 81-83 (in Ukrainian).
 
23. Kalinichenko, S.V., Babych, E.M., Kivva, F.V., Ryabovol, E.V., Gorbunov, L.V., Ryzhkova, T.A., Sklyar, N.I., Kovalenko, O.I., Katrych, V.A., Zhdamarova, L.A., Bobyreva, I.V., Kalinichenko, E.O., 2011. The influence of physical and physico-chemical factors on immunobiological properties of diphtheria toxin. Annals of Mechnikov Institute, 4, pp. 316-320 (in Ukrainian). Available from: http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?I21DBN=L...
 
24. Kalinichenko, S.V., Babich, E.M., Kuchma, I.Yu., Volyansky, Yu.L., Sklyar, N.I., Krestetska, S.L., Drach, M.I., Kolokolova, O.B., Rudenko, L.M., Kivva, F.V., Kovalenko, O.I., 2007. Method for modulation of microorganisms adhesive properties. Ukraine. Pat. 79848 (in Ukrainian).